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Indexes were added in a subsequent PCR (98 C for 30 sec, 8 (98 C for 10 s, 55 C for 20 s, 72 C for 20 s), 72 C for 5 min) using the Illumina Nextera Index Kit V2 and the attachments were verified by running the products on an agarose gel

Similar facial changes have long been observed after bariatric surgery and other forms of rapid, major weight loss, which is why Ozempic face is not unique to Ozempic