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Along with decreasing oxidative stress in tumours, Nrf2 activation increases the expression of downstream genes FSP1 and GPX4 , which can shield cancerous cells from iron ptosis [55, 56]

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The samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose (NC) membranes (GE Healthcare)

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