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60% (v/v) Percoll purification was performed as described above

Saffron and crocin ameliorate prenatal valproic acid-induced autistic-like behaviors and brain oxidative stress in the Male offspring rats

This is distinct from the native ligand ghrelin, which activates GHS-R1a promiscuously across multiple pituitary cell types and hypothalamic nuclei

GLP-1 strongly promoted cAMP accumulation but did not stimulate insulin secretion in low-glucose (2 mM) state, indicating glucose-induced signaling as the main pathway (Fig