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Warehouses MT-1 (Melanotan I, Afamelanotide, CAS 75921-69-6) is a synthetic linear tridecapeptide analog of alpha-melanocyte-stimulating hormone (-MSH) with two targeted amino acid substitutions norleucine at position 4 replacing methionine and D-phenylalanine at position 7 replacing L-phenylalanine supplied by Nextday Peptides in lyophilized format for laboratory and analytical research applications

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The temperature and IPTG concentration for protein expression were consistent with the above conditions, and ncAA p-acetylphenylalanine (pAcF) with a final concentration of 1 mM was added