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For the extraction, methanol and internal standards were added, followed by incubation for 15 min at 30C on a thermal shaker (650 rpm, TS1 ThermoShaker, Biometra, Gttingen, Germany), centrifugation (21,000 x g, 10 min, 4C, Heraeus Fresco 21 Centrifuge, Thermo Scientific, Schwerte, Germany), evaporation, and centrifugation with methanol again

Ahsan Shoeb, Jesper Ole Clausen, Birgitte Andersen, and Anna Bille Larsen, Novo Nordisk

Mice were monitored for clinical abnormalities ( Supplemental Table 6 )

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