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In vivo model included diverse organisms such as the nematode Caenorhabditis elegans ( Arabidopsis thaliana leaf infiltration model ( Galleria mellonella larvae ( Artemia salina ( In vitro studies using human cell lines have been extensively used to study pyocyanin, including NCI-H292 pulmonary carcinoma cells, 16HBE and NHBE (human bronchial epithelial cells), and A549 lung carcinoma epithelial cells ( in vitro studies provide controlled insights into cellular mechanisms and therapeutic effects, in vivo animal models with an intact immune system offer a comprehensive understanding of the biological impact of pyocyanin

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