glutathione reductase sigma aldrich disulfide reductase Glutathione Reductase ammonium sulfate suspension,

(2015) Methods Cell Biol

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After washing steps, DNA was eluted twice with elution buffer (25 mM TrisHCl pH 7.5, 5 mM EDTA pH 8.0, 0.5% SDS, 65 C, 20 min), treated with 10 mg/mL RNase-A (65 C, 1 h) and with 20 mg/mL proteinase K (65 C, 5 h), then was purified using the AxyPrep DNA Purification Kit (Axygen, AP-GX-250)