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Cytotoxicity Assay The cytocompatibility of CF- and CA-loaded liposomes, as well as the combined formulation (CF-CA-L), was assessed using the HaCaT human keratinocyte cell line (AddexBio) via the AlamarBlue assay

It is postulated that the skin lightening effect by cysteamine is due to its inherent antioxidant properties. The antioxidant also inhibits the melanin synthesis by decreasing the formation of dopachrome (the immediate precursor of eumelanin) and increasing intracellular glutathione, which shifts the eumelanin to pheomelanin synthesis, as well as to chelating copper ions required in melanogenesis

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Clinical evidence: in a cohort study involving 4,125 elderly individuals, higher cereal-derived RS intake (15 g/day) was associated with a 28% reduction in serum IL-6 levels and a 35% reduction in TNF- levels compared to low-RS intake ( 90%sufficient to meet 25% of daily protein requirements and support tryptophan (serotonin precursor) and tyrosine (dopamine precursor) supply (304)