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Some of the discrepancy comes from individual biology

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and 0.33g of compound 1 was dissolved in 100mL of acetonitrile, and then added dropwise to the above mixture to react at 0 to 5 for 3 hours, the resulting reaction solution was adjusted to pH 6.0 with phosphoric acid, and then 2 times of purified water was added, and centrifugation was carried out to obtain solid compound 2, the liquid chromatogram of FIG