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The gene was subcloned into the BacMam vector 60 , with a C-terminal eGFP and PreScission cleavage site, using primers (Fwd: CCACTCCCAGTTCAATTACAGCTCTTAAGGGCCACCATGCGGGACTACGACGAGG and Rev: CAGCACTTCCAATCTAGATTCGAAAGCGGCGCCGAAGGCTGTGCTTTTAAGGATT

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Methionine converts to S-adenosylmethionine (SAMe) to facilitate fat metabolism, inositol supports insulin signaling, and choline prevents hepatic fat accumulation

127 Another clinical trial with 180 participants compared a combination of vitex (40 mg), soy isoflavones (60 mg), magnolia (50 mg), Lactobacillus sporogenes (109 spores [the dormant form of this bacterium]), and vitamin D (35 mcg or 1,400 IUs) to soy isoflavones alone for 12 months and found the combination formula was more effective for reducing frequency and intensity of hot flashes, and improving sleep and psychological wellness