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is supported by the NIHR Newcastle Biomedical Research Centre and the Innovative Medicines Initiative (IMI2) program of the European Union

Real-time quantitative PCR, triglycerides, and Oil Red O staining Intramuscular preadipocytes isolation and plasmid construction Intramuscular preadipocytes of the longissimus dorsi muscle were isolated from calves of Qinchuan cattle at National Beef Cattle Improvement Center Breeding Farm using 0.2% type II collagenase (1148090, Sigma), following the protocol described in our laboratorys previous studies [30,31,32]

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Look beyond the weight loss to maintain health during this self-starving process