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Considering that this compound has been demonstrated to expand the GSH system along with a reduction in oxidative injury by the AKT/Nrf2 signaling pathway in the hearts of rats and H9C2 cells, the abovementioned enhanced TAC may cover GSH and GSH-dependent antioxidant enzymes [150,151,152]

Add the elution buffer to the column, cap both ends, and incubate for a few minutes before uncapping and dripping your elution out of the bottom (Figure 4)

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