semaglutide recombinant synthesis Enzymatic Strategies Behind Manufacturing The 6 Billion Dollar Synthesis

The compound of formula- 10 which is used in the present invention can be prepared by a process comprising: a) treating compound of formula-20 with compound of formula-21 in presence of a solvent and/or a base optionally in presence of an alkali metal halide to provide compound of formula-22, Formula-21 wherein, X represents halogens such as Cl, Br and I

The fermentation may be carried out in fed-batch to produce expressed semapeptide

la rsine 2-CTC a un degr de substitution de 0,2 0,6 mmol/g

The process as claimed in claims 1 and 6, wherein the conversion of a peptide having amino acid sequence His-Aib-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Val-Ser-Ser-Tyr-Leu-Glu- Gly-Gln-Ala-Ala-Lys-Glu-Phe-Ile-Ala-Trp-Leu-Val-Arg-Gly-Arg-Gly to Semaglutide is carried out by global deprotection of the compound to cleave the peptide chain from the Resin and deprotection of the protected amino acids by using cocktail mixture/cleaving reagent and reacting the obtained compound with compound of formula-3 in a solvent optionally in presence of a base and/or a coupling agent