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Following incubation, beads were washed once with cold KPBS (pH=7.25) before being incubated in 200 l transport buffer (KPBS+10 mM HEPES + 0.5 mM EGTA) at room temperature containing indicated doses of [ 13 C 2 , 15 N]-GSH (Cambridge Isotope Laboratory, CNLM-6245-50) for the indicated times

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Transcriptomic analysis of High ADA expressing cells shows increased expression of P2 receptors, Inflammatory cytokines and osteoclast markers We further validated the in vitro experiments on RAW 264.7 cells, showing elevated cytokine expression in the presence of ADA is of consequence in vivo