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Second, we hypothesized that chronic, exogenous activation of this excitatory pathway could reverse the crossed cerebellar diaschisis, and possibly even atrophic changes, that occur following contralateral cortical ischemia and contribute to loss of function [90, 94, 120]

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4.5 Live cell imaging Confocal images of 4 optical planes interspaced by 1 m (from -1.0 m to 2.0 m included) across 25 x 25 fields of view with a 10% overlap were acquired per well on an Opera Phenix High Content Screening System (PerkinElmer) using a 40x or 63x/1.15 NA water-immersion objective, with live cell parameters (37 C and 5% CO 2 )

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