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However, the absence of cysteine leads to insufficient GPX4 synthesis, increased expression of wild-type p53, and increased cell sensitivity to ferroptosis (Ji et al., 2021)

The three libraries were generated using error-prone PCR as described above, using the following conditions to produce varying levels of mutagenesis: Library 1: 2 M 8-oxo-dGTP, 0.5 M dPTP, 20 PCR cycles Library 2: 2 M 8-oxo-dGTP, 1 M dPTP, 20 PCR cycles Library 3: 2 M 8-oxo-dGTP, 2 M dPTP, 20 PCR cycles The library sizes (approximated by number of transformants as described above) were 6 10 7 for each library

doi: 10.1097/mca.0000000000000830 13 SilverHJOlsonDMayfieldDWrightPNianHMashayekhiMet al