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46 Previous studies reported that GSH concentrations consistently decreased in the serum, erythrocytes, and CSF in patients with MS 14 , 17 and have stimulated numerous studies to develop new potential approaches to maintain or restore GSH levels in these patients
Since INS-1E cells are rat-derived, we used the mRNA sequence encoding rat 2A -adrenergic receptor as a template to generate guide RNA (gRNA) to disrupt the promoter region of the Adra2a gene (gRNA sequence: 5-GCAGCCGGATGCCGGCAATA-3, positions 5776)
Insulin resistance is an important driver of metabolic disease, yet the molecular changes in insulin signalling and/or GLUT4 traffic that confer insulin resistance remain largely unknown
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