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The binding affinity of GHK for copper(II) is extremely high in the picomolar range for the apparent dissociation constant at physiological pH which means that in the presence of physiological copper, free GHK and copper-bound GHK readily interconvert with the bound form dominating

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[DOI] [PubMed] [Google Scholar] 39.Yu W., Daniel J., Mehta D., Maddipati K.R., Greenberg M.L

Briefly, cells were lysed in PBS containing 1% Triton and 1 mM phenylmethylsulfonyl fluoride (PMSF), along with a complete set of EDTA-free protease inhibitors (Thermo Scientific, Rockford, IL, USA)