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Seven modified cysteine sites were confirmed, including Cys112 in GSTA1, Cys78 in GSTM1, Cys115 and 174 in GSTM2, as well as Cys15, 48, and 170 in GSTP1

The oocytes were then incubated at room temperature in blocking buffer (1% BSA/PHEM, 100 mM glycine), followed by overnight incubation at 4 C with the primary antibody

Advanced peptide mapping and HRMS methods are often necessary for accurate identification

However, it is important for patients to discuss potential side effects and interactions with their healthcare provider, as gabapentin can cause dizziness, fatigue, and other adverse reactions in some individuals