For virus infection, HT1080 cells were spin infected in 12-well tissue culture plates using 8 g/ml of polybrene at 1200 g for 60 min and selected by puromycin (0.5 g/ml) or blasticidin (4 g/ml) for 3 days
Common side effects include gastrointestinal symptoms such as nausea, vomiting, and diarrhea
doi: 10.3892/or.2015.4086
(Protexin ), Lopen Head, Somerset, UK] and one 250 mg capsule/day after lunch containing 175 mg fructo-oligosaccharide (FOS), plus 1 10 8 colony-forming unit (CFU)/capsule, freeze-dried Protexin probiotics, including Bifidobacterium breve PXN 25 , Bifidobacterium longum PXN 30 , Lactobacillus casei PXN 37 , Lactobacillus rhamnosus PXN 54 , Lactobacillus acidophilus PXN 35 , Lactobacillus bulgaricus PXN 39 , and Streptococcus thermophilus PXN 66 , while those in the L-carnitine + placebo group received the same amounts of L-carnitine and 250 mg maltodextrin capsule/day, as the synbiotic placebo (FIC Co., China), for eight sequential weeks