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After TMB substrate solution is added, only those wells that contain Human Flt3L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

Park P-J, Moon B-S, Lee S-H, Kim S-N, Kim A-R, Kim H-J, Park W-S, Choi K-Y, Cho E-G, Lee TR (2012) Hair growth-promoting effect of Aconiti Ciliare Tuber extract mediated by the activation of Wnt/-catenin signaling

After opening, best if consumed within 30 days

Later, this definition was extended to include essentially similar protective effects outside the gastrointestinal tract involving the lesions of different organs (cytoprotection-organoprotection)