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Upon loss of microbial signals, modeled by germ-free, gnotobiotic, and broad-spectrum antibiotic-treated rodents, eEANs sensed the changes in intestinal epithelial and mucosal areas in the lamina propria, relayed signals to sensory vagal afferents of the nodose ganglion and dorsal vagal complex, sympathetically activated glutaminergic neurons lateral to the paragigantocellular nucleus/rostral ventrolateral medulla (LPGi/RVLM) and downstream coeliac-superior mesenteric ganglia (CM-SMG), whereby efferent nerves reduced GI transit time