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RNA extraction and RTqPCR Total RNA from cells was isolated via TRIzol (Takara, Otsu, Japan) and subsequently purified with chloroform/isopropanol/ethanol, and cDNA was synthesized via the PrimeScript RT reagent Kit (Takara) according to the manufacturers instructions and used as the template for qRTPCR

Massage it till it's fully absorbed.

We examined the epidermal thickness and detected the expression of K16, K17, and Ki67 to assess the proliferation of mouse skin keratinocytes, as in previous mouse experiments

Mitochondrial abnormalities are another hallmark of cystinosis, with studies demonstrating reduced ATP production and evidence of metabolic imbalances [144, 146, 148]