GLUTATHIONE IMPORT TO MITOCHONDRIA As indicated above, despite the fact that the concentration of mGSH is high, GSH is not synthesized de novo in the mitochondrial matrix, as this organelle lacks the enzymes required for GSH synthesis

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Vascular endothelial growth factor (VEGF), a typical marker of angiogenesis during wound healing, was quantitatively assessed via ELISA on day 3, revealing a significant increase in the G/D-CuP group (Fig
Considering that these genes are essential, as evidenced by comparing the sgRNA abundance in non-treated cells with the library plasmid stock (Fig S2B), the observed phenotype is most likely unrelated to metal toxicity