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The extant literature provides compelling evidence that an individual's dietary habits, exercise regimen, and sleep patterns play a pivotal role in the efficacy of a given treatment [33, 34]

The efficient knockdown of both Barr1 mRNA and Barr1 protein was confirmed by real-time reverse transcription PCR (qRT-PCR) (Figure 4C) and immunoblotting studies (Supplemental Figure 15), respectively