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Shalev et al., 2001

An appropriate amount of the cell suspension was then aspirated using a pipette and transferred into the counting chamber of a hemocytometer, after which erythrocytes were counted under an optical microscope

All four pairs of BsNbs exhibited the corresponding BsNb peak in chromatogram, and the conjugation efficiency was greater than 50% (Table S5)

This difference is due to a single amino acid at the 2nd position in the N-terminal, where GLP-1 has an alanine (Ala8) while exendin-4 has a glycine (Gly2)