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This study discussed the changes of metformin on 4EBP1, S6K1, and mTOR in colon cancer cells from the mRNA level and protein level, and confirmed that metformin can further promote cell apoptosis by inhibiting the activity of the mTOR signaling pathway and inhibiting the levels of 4EBP1 and S6K1, showing an obvious dose-dependent effect (58)

PubMed Central , Archives of Medical Science : AMS , vol

But for people with active cancer, a prior cancer diagnosis, or ongoing cancer treatment, NAC is something to discuss with an oncologist or healthcare provider before use

Determination of melanin content The total concentration of melanin produced by the treated cells was calculated as a previously reported method by Tsuboi et al