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Repeated freeze-thaw cycles destroy peptides through ice crystal formation that physically tears apart molecular structures

5 This can have significantly detrimental effects on the nervous system, cell division, and other methylation processes

The involvement of bombesin-like peptides in the pathogenesis of a wide range of human tumors, their function as autocrine/paracrine tumoural growth factors, and the high incidence of BN/GRP receptors in various human cancers prompted the design and synthesis of BN/GRP receptor (GRPR) antagonists such as RC-3095, RC-3940-II, and RC-3950 [136138]

Using distilled water, tap water, or saline introduces contaminants or changes the solution chemistry in ways that can damage the peptide or cause adverse reactions