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mRNA expression analysis by real-time PCR Total RNA was isolated from the dissected tissues using the RNeasy Mini kit (Qiagen, CA, USA), followed by reverse transcription using the High Capacity RNA-to-cDNA kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturers instructions

and broad transcriptional effects, with gene-expression analyses identifying modulation of expression patterns associated with antioxidant defense, DNA repair, and tissue remodeling

En la enca se ha observado una lesin similar en los fumadores, con aumento del pigmento melnico en la zona vestibular, gingival de caninos inferiores

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