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Semaglutide in obesity-related heart failure with preserved ejection fraction and type 2 diabetes across baseline HbA(1c) levels (STEP-HFpEF DM): a prespecified analysis of heart failure and metabolic outcomes from a randomised, placebo-controlled trial

The mice were injected intravenously with PBS, Ru(II)-OH, NP2, NP4 (2 mg/kg Ru) on days 1, 4, 7, 10, respectively

And the tissue sections were incubated with primary antibodies overnight and HRP-conjugated secondary antibody for 1 h, following stained with haematoxylin for 8 min