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A probable reason for the variability could be the number of bacteria transplanted, and therefore, further research should be conducted to determine the most appropriate levels of bacteria that should be used in FMT to achieve the appropriate decrease in TMAO [41]

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Lung tissue and sample preparation In each group, the left lungs of 6 mice were infused with 0.5 ml PBS three times for the harvest of bronchoalveolar lavage (BAL) fluid, while the left lung tissues of the other 6 mice were inflated with 10% neutral formaldehyde at positive pressure (25 cm H 2 O) ( Histology The paraffin sections (4 m) were stained with hematoxylin and eosin (H&E)