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This finding suggested the possibility that acute stress protection is at least partially based on persulfide generation

Reaction was finished by addition of detection mix (provided with the assay kit), plates were mixed for 30 minutes and then rested for three hours at RT before counting in a TopCount (Perkin Elmer)

In vivo delivery of NanoLuc expressing plasmid DNA To evaluate DNA delivery efficiency, luciferase (Luc) activity was measured in major organs (liver, lung, spleen, kidney and heart) of 6-8 weeks-old female Balb/c mice

IV delivery ensures maximum absorption, which is particularly important when oral intake is compromised by vomiting