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To further explore potential differences in how men and women respond to the treatment, researchers conducted a post-hoc analysis of the SURMOUNT (SM) program, which included four clinical trials (SM-1 to SM-4)

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The cells were analyzed with the Seahorse XFe24/96 Extracellular Flux analyzer (Agilent) to determine real-time oxygen consumption rate and extracellular acidification rate and normalized the Seahorse measurements by counting cell number with Biotek citation 1/5, as previous reported 7