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The elevated mechanical tension that resulted from a stiffened ECM diminished AT2 to AT1 differentiation and increased the abnormal accumulation of PATS

Precipitates were then washed with ice-cold water, lysed with 0.5 M NaOH and mixed with liquid scintillation counter cocktail (Optiphase Supermix, PerkinElmer)

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This would likely occur shortly after the injection (within minutes to an hour)