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In accordance with these changes in the microbiota composition after CIA induction, butyrate levels changed, dropping just before the onset of arthritis indicative for a changed microbiota composition and decreased function of the intestinal barrier 25 (Supplementary Fig

Amplification cycles were: initial denaturation at 94 C for 3 min, followed by 35 cycles of denaturation at 94 C for 30 s, annealing at 55 C for 30 s, and extension at 72 C for 1 min, followed by a final extension at 72 C for 7 min