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If you shook it vigorously for an extended period, some degradation may have occurred

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This approach has the advantage that it integrates the effect of both genetic background and nutritional status of the cells in evaluating disease susceptibility

These may occur if: The injection was too shallow or too deep The fluid volume was too high for the site The product was too concentrated or acidic Local immune cells formed a small granuloma (harmless but annoying) Applying a warm compress can help disperse the fluid and reduce the lump