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Endogenous ergothioneine levels were shown to increase with exercise in mouse skeletal muscle, resulting in its increased binding and activation of the H 2 S-generating enzyme MPST, resulting in increased mitochondrial ETC function and increased exercise endurance (Sprenger et al., 2025), as the H 2 S generated by the metabolism of endogenous skeletal muscle ergothioneine was shown to increase mitochondrial ETC activity through the transfer of electrons from H 2 S to ubiquinone (Landry et al., 2018)

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