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At the conclusion of the 28-day treatment period, the efficacy of each treatment was assessed and mice were humanely euthanized using CO 2 gas

Indeed, direct tests revealed a lack of input specificity of synaptic changes in CA1 inhibitory neurons (McMahon and Kauer, Thus, initial studies demonstrate that CA1 interneurons can express long-term synaptic plasticity, which is calcium-dependent (Cowan et al., 2+ imaging has demonstrated that synaptically induced rises of [Ca 2+ ] i in aspiny dendrites do not spread much but are kept local by interneuron-specific mechanisms (Goldberg et al., Box 1 and below in the sections on calcium signals and heterosynaptic plasticity

Much of the foundational research also comes from a single research group, which limits the breadth of independent validation