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The green bark tissues within 0.51.0 cm of the injury site were shortly placed in liquid nitrogen and stored at 80C

Cells were imaged in fresh FluoroBrite DMEM supplemented with 4 mM glutamine, 1 mM sodium pyruvate, 10% FBS and penicillin (100 U ml 1 )/streptomycin (100 g ml 1 )/fungizone (0.25 g ml 1 )

Endocrinology 152, 43224335

Moreover, recently it has been reported that the pathway governed by NE2FL2 is not only regulated by the proteasome but also by ATG (Jain et al., 2013)