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IL-12 facilitates CD4+ T-cell differentiation into Th1 cells that secrete elevated levels of interferon-gamma (IFN-), which subsequently activates NK cells and cytotoxic T lymphocytes (CTLs), thereby enhancing their anti-tumoral functions ( Furthermore, IL-12 diminishes regulatory T-cell (Treg) and myeloid-derived suppressor cell (MDSC) populations within tumoral environments alleviating suppression mechanisms detrimental toward effective antitumoral responses ( Moreover, IL-12 sensitizes neoplasms toward checkpoint inhibitors like PD-1/PD-L1 blockade, thereby amplifying therapeutic efficacy ( 4 Others In addition to the aforementioned wide-ranging applications in genetic modification techniques, several strategies for genetic modification demonstrate significant potential for clinical application ( Table 1 )