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The effects of GHK and GHK-PEG on malignant cell proliferation was first tested on U937 cells which were plated in 24 well plates and treated with GHK or PEGylated GHK at 10 and 20 nM for 24 hours and cell proliferation was assessed using a hemocytometer and the effect of each treatment was compared between control and treated samples using analysis of variance (ANOVA)

Figure 1 Perfusion of GLP1 (100 nM) increased the membrane excitability of PCs, which was manifested by a significant increase in the frequency of spike firing discharge elicited by depolarization current, accompanied by significant depolarization of membrane potential (Figure 2A)

A 2002 study published in the Bulletin of Experimental Biology and Medicine found that nanomolar concentrations increased biosynthetic activity in hepatocyte cultures from old rats, restoring circadian rhythms of protein production that had been disrupted by aging

Several serious health concerns are directly related to the use of this medication, especially for pregnant women and children