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An initial approach to the quantification of total tissue polysulfides was to use a phosphine that incorporates the sulfane sulfur atom into a diagnostic derivative, in conjunction with isotope dilution and liquid chromatography tandem mass spectrometry (LC-MS/MS) 31,32
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The kit works well, and I would recommend a total loading amount of 2g protein of lysate for good performance
Other serious but less common risks include pancreatitis (inflammation of the pancreas), gallbladder problems, and kidney problems
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