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Degeneration pattern of human first-order cochlear neurons

Frostbite occurs when tissues are exposed to cold temperatures, commonly resulting in freezing on the extremities and can result in the need for amputation or cause tissue failure and impairment, associated with the development of free radicals, which cause additional tissue damage

For the proteomic analysis, 300 L aliquots of urine were centrifuged twice at 10,000 g for 20 min at 4C to separate urinary proteins from small molecules using an Amicon Ultra 0.5 ml Centrifugal Filter (10 kDa, UFC501096, Merck Millipore, Darmstadt, Germany) according to the manufacturers instructions