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In another study using HT22 cells, huperzine A (HupA) + wortmannin treatment stopped the downregulation of p-mTOR, p-Akt, and p-p70s6 kinase under oxidative glutamate toxicity, leading to the conclusion that HupA abrogates oxidative glutamate toxicity in mouse hippocampal cells via the activation of the BDNF/TrkB-dependent PI3K/Akt/mTOR signaling pathway 51

Therefore, SARS-CoV-2 infection seems to affect -synuclein and death of dopaminergic neurons, which is known to cause PD

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MDA measurement Approximately 1 mL of pre-cooled extraction solution was added to prepare the cell lysate, followed by 30 cycles of sonication