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The resulting protein was centrifuged to remove any aggregates and loaded onto a Superdex 200 16/600 size exclusion chromatography (SEC) column equilibrated in SEC buffer for crystallisation (10 mM Tris-HCl pH 8.0, 200 mM KCl, 0.5 mM PMSF, 1 mM TCEP) 31 or NMR (50 mM mono/dibasic sodium phosphate buffer pH 8.0, 150 mM NaCl, 1 mM TCEP)

The increase in YI might be due to an improvement in the hepatic biosynthesis rate of yolk precursors and/or an alteration in the mode of their transport from the liver into the ovarian follicle and oocyte (Rabie et al., 1997)

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Sinha R, Sinha I, Calcagnotto A, et al Oral supplementation with liposomal glutathione elevates body stores of glutathione and markers of immune function Eur J Clin Nutr