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Isolated hepatocytes were plated on collagen-coated 6-well plates (4 10 4 cells per cm 2 ) in DMEM (Gibco #12800-017, containing 1 mM pyruvate, 25 mM glucose, and 4 mM glutamine) supplemented with 10 % (vol/vol) fetal bovine serum (FBS), 100 units/ml penicillin, 100 g/ml streptomycin, 1 M dexamethasone, 0.1 M insulin, and an additional 1 mM sodium pyruvate and incubated at 37 C in an atmosphere of 5 % CO 2

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Subsequently, 10 L of CCK8 (APExBIO) solution was added to each well and incubated for 3 h, then the absorbance value (OD) was measured at 450 nm

Research in this area is preliminary, and is still ongoing