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On attending the administering nurse will greet you and once in the treatment room ask you some basic health questions, again check your screening form and check that you consent to proceed with the planned injection

Pulldown assay Wild-type (WT) and mutant GSTs were expressed in Rosetta (DE3) competent cells by 1 mM isopropyl -D-1-thiogalactopyranoside (IPTG) (Amnesco)

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NF-B (p65) DNA-binding activity analysis NF-B (p65) DNA-binding activity in the nuclei of homogenized liver tissues or cultured cells was measured using ELISA kits (Cayman, MI, USA) in accordance with the manufacturers instructions