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4.5 Live cell imaging Confocal images of 4 optical planes interspaced by 1 m (from -1.0 m to 2.0 m included) across 25 x 25 fields of view with a 10% overlap were acquired per well on an Opera Phenix High Content Screening System (PerkinElmer) using a 40x or 63x/1.15 NA water-immersion objective, with live cell parameters (37 C and 5% CO 2 )

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Since B12 is water-soluble, meaning your body easily pushes out any extra through urine once youve absorbed what you need, there is no concern of overdosing on B12

Species Several ASVs mapped with high confidence directly to a specific species (Supplementary Table S4), with considerably more species mapped using the BLASTn method compared to the BLCA method