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Membranes were blocked with 5% non-fat milk and incubated with mouse anti-5mC (#61480, Active motif) or anti-5hmC (#39999, Active motif) followed by a horseradish peroxidase-conjugated antibody (Santa Cruz Biotechnology)

Introduction The growth hormone-insulin-like growth factors (GH-IGFs) growth axis plays widely roles in varieties of systems, including nervous system, reproductive system, skeletal system, muscular system, and immune system (Wu and Gao, 2018), indicating that the effects of GH and IGF1 on cell proliferation can not only maintain the growth and development of normal organism, but also promote the process of cancer cells

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doi: 10.1007/s00431-002-1132-4 74 BrunettiGDAmatoGChiaritoMTulloAColaianniGColucciSet al