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Lysates were diluted in 2 Laemelli SDS sample buffer containing 50 mM DTT to equal concentration then ~ 10 g of protein electrophoresed through NuPage 38% TrisAcetate gels (Thermofisher Scientific) alongside Kaleidoscope (Bio-Rad, UK) and HiMark (Invitrogen, UK) protein standards

In euthyroid individuals, the thyroid gland has compensatory homeostatic mechanisms, known as the Wolff-Chaikoff effect, that prevent excess production of thyroid hormone following an iodine load, with the thyroid gland readjusting within days or weeks

In more severe cases, individuals might experience more intense reactions that require medical attention

M., Rodriguez-Palero, M